Papers

Publications

1. Parichanon P., Ascrizzi R., Tani C., Sanmartin C., Taglieri I., Macaluso M., Flamini G., Pieracci Y., Venturi F., Conti B. – The protective combined effect of chitosan and essential oil coatings on cheese and cured meat against the oviposition of Piophila casei. Food Biosci., 2023, 103132. https://doi.org/10.1016/j.fbio.2023.103132.
2. Calabretta M.M., Gregucci D., Michelini E. – New synthetic red- and orange-emitting luciferases to upgrade in vitro and 3D cell biosensing. Analyst 2023, Advance Article. https://doi.org/10.1039/D3AN01251D.
3. Karrat A., García-Guzmán J.J., Palacios-Santander J.M., Amine A., Cubillana-Aguilera L. – New strategies for the removal of template from the ion and molecularly imprinted polymers: Application to the fast and on-site Cr(VI) detection with a smartphone. Sensors and Actuators B: Chemical 2023, 386, 133751. https://doi.org/10.1016/j.snb.2023.133751.
4. Gregucci D., Nazir F., Calabretta M.M., Michelini E. – Illuminating progress: The contribution of bioluminescence to Sustainable Development Goal 6-Clean Water and Sanitation-of the United Nations 2030 Agenda. Sensors 2023, 223, 7244. https://doi.org/10.3390/s23167244.
5. Martínez-Pérez-Cejuela H., Calabretta M.M., Bocci V., D’Elia M., Michelini E. – Super-Stable Metal-Organic Framework (MOF)/Luciferase Paper-Sensing Platform for Rapid ATP Detection. Biosensors 2023, 13, 451. https://doi.org/10.3390/bios13040451.
6. Calabretta M.M., Gregucci D., Desiderio R., Michelini E. – Colorimetric paper sensor for food spoilage based on biogenic amine monitoring. Biosensors, 2023; 13, 126. https://doi.org/10.3390/bios13010126.
7. Farina P., Ascrizzi R., Bedini S., Castagna A., Flamini G., Macaluso M., Mannucci A., Pieracci Y., Ranieri A., Sciampagna M.C., Venturi F., Conti B. – Chitosan and essential oils combined for beef meat protection against the oviposition of Calliphora vomitoria, water loss, lipid peroxidation, and colour changes. Foods, 2022, 11, 3994. https://doi.org/10.3390/foods11243994.
8. Calabretta M.M., Gregucci D., Martínez-Pérez-Cejuela H., Michelini E. – A luciferase mutant with improved brightness and stability for whole-cell bioluminescent biosensors and in vitro biosensing. Biosensors (Basel), 2022, 12, 742. https://doi.org/10.3390/bios12090742.
9. Martínez-Pérez-Cejuela H., Gregucci D., Calabretta M.M., Simó-Alfonso E.F., Herrero-Martínez J.M., Michelini E. – Novel nanozeolitic imidazolate framework (ZIF-8)-luciferase biocomposite for nanosensing applications. Anal. Chem., 2022, in printing. https://doi.org/10.1021/acs.analchem.2c05001.
10. Calabria, D., Zangheri, M., Pour, S.R.S., Trozzi, I., Pace, A., Lazzarini, E., Calabretta, M.M., Mirasoli M., Guardigli M. – Luminescent aptamer-based bioassays for sensitive detection of food allergens. Biosensors (Basel), 2022, 12, 644. https://doi.org/10.3390/bios12080644.
11. Calabretta M.M., Gregucci D., Guarnieri T., Bonini M., Neri E., Zangheri M., Michelini E. – Bioluminescence sensing in 3D spherical microtissues for multiple bioactivity analysis of environmental samples. Sensors (Basel), 2022, 22, 4568. https://doi.org/10.3390/s22124568.
12. Karrat A., Palacios-Santander J.M., Amine A., Cubillana-Aguilera L. – A novel magnetic molecularly imprinted polymer for selective extraction and determination of quercetin in plant samples. Analytica Chimica Acta, 2022, 1203, 339709. https://doi.org/10.1016/j.aca.2022.339709.
13. Tampucci S., Castagna A., Monti D., Manera C., Saccomanni G., Chetoni P., Zucchetti E., Barbagallo M., Fazio L., Santin M., Ranieri A. – Tyrosol-enriched tomatoes by diffusion across the fruit peel from a chitosan coating: a proposal of functional food. Foods, 2021, 10, 335. https://doi.org/10.3390/foods10020335.
14. Calabretta M.M., Alvarez-Diduk R., Michelini E., Merkoçi A. – ATP sensing paper with smartphone bioluminescence-based detection. Methods in Molecular Biology, 2022, 2525, 297-307. https://doi.org/10.1007/978-1-0716-2473-9_23.
15. Lopreside A., Montali L., Wang B., Tassoni A., Ferri M., Calabretta M.M., Michelini E. – Orthogonal paper biosensor for mercury(II) combining bioluminescence and colorimetric smartphone detection. Biosensors and Bioelectronics, 2021, 194, 113569. https://doi.org/10.1016/j.bios.2021.113569.
16. Karrat A., Amine A. – Solid-phase extraction combined with a spectrophotometric method for determination of Bisphenol-A in water samples using magnetic molecularly imprinted polymer. Microchemical Journal, 2021, 168, 106496. https://doi.org/10.1016/j.microc.2021.106496.
17. Attaallah R., Amine A. – The kinetic and analytical aspects of enzyme competitive inhibition: sensing of tyrosinase inhibitors. Biosensors 2021, 11, 322. https://doi.org/10.3390/bios11090322.
18. Ascrizzi R., Flamini G., Bedini S., Tani C., Giannotti P., Lombardi T., Conti B., Fraternale D. – Ferulago campestris essential oil as active ingredient in chitosan seed-coating: chemical analyses, allelopathic effects, and protective activity against the common bean pest Acanthoscelides obtectus. Agronomy, 2021, 11, 1578. https://doi.org/10.3390/agronomy11081578.
19. Calabretta M.M., Lopreside A., Montali L., Cevenini L., Roda A., Michelini E. – A genetically encoded bioluminescence intracellular nanosensor for androgen receptor activation monitoring in 3D cell models. Sensors, 2021, 21, 893. https://doi.org/10.3390/s21030893.
20. Calabretta M.M., Zangheri M., Calabria D., Lopreside A., Montali L., Marchegiani E., Trozzi I., Guardigli M., Mirasoli M., Michelini E. – Paper-based immunosensors with bio-chemiluminescence detection. Sensors, 2021, 21, 4309. https://doi.org/10.3390/s21134309.
21. Calabretta M.M., Montali L., Lopreside A., Fragapane F., Iacoangeli F., Roda A., Bocci V., D’Elia M., Michelini E. – Ultrasensitive on-field luminescence detection using a low-cost silicon photomultiplier device. Analytical Chemistry, 2021, 93, 7388-7393. https://doi.org/10.1021/acs.analchem.1c00899.
22. Farina P., Venturi F., Ascrizzi R., Flamini G., Chiriboga Ortega R.D., Echeverría M.C., Ortega S., Zinnai A., Bedini S., Conti B. – Andean plants essential oils: a scented alternative to synthetic insecticides for the control of blowflies. Insects, 2021, 12, 894. https://doi.org/10.3390/insects12100894.
23. Farina P., Abenaim L., Conti B. – Enemies and allies: the different roles of insects towards fruit and vegetable loss and waste. Agrochimica, 2021, 65, Special Issue “180 years of excellence in agricultural sciences in Pisa: The state of research today”, 9-16. https://doi.org/10.12871/00021857202202.
24. Bedini S., Farina P., Napoli E., Flamini G., Ascrizzi R., Verzera A., Conti B., Zappalà L. – Bioactivity of different chemotypes of oregano essential oil against the blowfly Calliphora vomitoria vector of foodborne pathogens. Insects, 2021, 12, 52. https://doi.org/10.3390/insects12010052.

Living labs:

1. Economia circolare e packaging sostenibile: il progetto FEDKITO. June 10^th, 2022, University of Ferrara and University of Pisa, Italy. Conti B., Farina P., Tani C., Abenaim L., Michelini E., Bartolini F.
2. Un living lab del progetto Fedkito: “Il contributo degli insetti alla sostenibilità”. May 17^th, 2023, Pisa, Italy. https://2023.festivalsvilupposostenibile.it/cal/131/un-living-lab-del-progetto-fedkito-il-contributo-degli-insetti-alla-sostenibilita.
3. Economie circulaire et emballage durable à base de chitosan et d’huiles essentielles. September 1^th, 2023, IPC (Technical Industrial Centre of the French plastics and composites)  Conti B., Venturi F., Khemakhem M., Michelini E. (facilitator Mazzoleni L.). Video link

Congresses:

1. SCI-Analitica 2023. Vasto, Italy, September 17-19^th, 2023. Gregucci D., Calabretta M.M., Michelini E. – Combining new luciferase mutants with metal-organic-frameworks to develop new biosensing tools.
2. IFHN-2023, 7^th Edition of Innovations in Food Science and Human Nutrition, July 19-20^st, 2023, Frankfurt, Germany. Ranieri A. – The role of chitosan for food preservation: a valuable polysaccharide derived from fishing and insect industry waste.
3. Book of Abstracts of the XXVII Italian National Congress of Entomology (CNIE 2023), Palermo (PA), Italy, June 12-16^th, 2023, 304. Farina P., Tani C., Bedini S., Venturi F., Ascrizzi R., Pieracci Y., Flamini G., Conti B. – Application of chitosan enriched with essential oils to protect fresh foodstuffs from the attack of dipteran pests.
4. Book of Abstracts of the XXVII Italian National Congress of Entomology (CNIE 2023), Palermo (PA), Italy, June 12-16^th, 2023, 28. Abenaim L., Caparros Megido R., Dallai R., Mercati D., Mandoli A. – Plastivorous activity of Hermetia illucens larvae (Diptera Stratiomyidae).
5. Barbara Conti1. – Application of chitosan enriched with essential oils to protect fresh foodstuffs from the attack of dipteran pests.
6. Biosensors 2023, 33^rd Anniversary World Congress on Biosensors, Busan, South Korea, June 5-8t^h, 2023. Michelini E., Martínez-Pérez-Cejuela H., Gregucci D., Simó-Alfonso E.F., Herrero-Martínez J.M., Calabretta M.M. – Novel nano zeolitic imidazolate framework (ZIF-8) – luciferase biocomposites for nanosensing applications (Poster).
7. Biosensors 2023, 33^rd Anniversary World Congress on Biosensors, Busan, South Korea, June 5-8t^h, 2023. Calabretta M.M., Mugnaini S., Michelini E. – Bioluminescent tissue on-a-chip for multiplexed biosensing.
8. Nenu2Phar H2020 project at IPC- Alençon, France, April 4^th, 2023. Poster by Quentin Blot.
9. Giornate di Bioanalitica 2023, Florence, Italy, March 27-28^th, 2023. Gregucci D., Calabretta M.M., Michelini E. – Smartphone-based colorimetric paper sensor for food quality control based on biogenic amines monitoring.
10. Giornate di Bioanalitica 2023, Florence, Italy, March 27-28^th, 2023. Calabretta M.M., Gregucci D., Bonini M., Neri E., Zangheri M., Michelini E. – Bioluminescence analytical method based on 3D microtissues for the evaluation of different bioactivities of water samples (Poster).
11. 6^th International Symposium on Medicinal and Aromatic Plants SIPAM, March 18-21^st, 2023, Djerba, Tunisia. Taglieri I., Sanmartin C., Farina P., Bedini S., Conti B., Ferroni G., Venturi F. – Essential oils combined with chitosan for beef meat preservation: a sensory analysis approach to improve a sustainable storage solution.
12. 5° Workshop – I chimici per le Biotecnologie, Naples, Italy February 27^th, 2023. Calabretta M.M., Michelini E. – Bioluminescence sensing in 3D spherical microtissues for multiply bioactivities analysis of environmental samples.
13. All4Pack Emballage, Paris Nord Villepinte, France, November 22^nd, 2022. Poster by Quentin Blot.
14. 4^th Edition of Analytical and Bionalaytical Conference, October 17-18^th, 2022. Calabretta M.M., Gregucci D., Michelini E. – Predictive in vitro sensing tools exploiting 3D spherical bioluminescence microtissues.
15. 3^rd World Conference Revitalization of Mediterranean Diet “Change of Route Towards More Sustainable and Resilient Food Systems in Mediterranean Countries: the Mediterranean Diet as a Strategic Resource to Accelerate the Agenda 2030 in the Region”. September 28-30^th, 2022, International Centre for Advanced Mediterranean Agronomic Studies (CIHEAN), Bari, Italy.
16. XL National Congress of the Italian Society of Agricultural Chemistry (SICA) Conciliating sustainability, resilience, and food quality. New challenges for a 2030 agriculture. September 5-7^th, 2022, Pisa, Italy. Mannucci A., Boschi A., Pomarè L., Santin M., Coltelli M.B., Panariello L., Abenaim L., Conti B., Castagna A., Ranieri A. – Extraction and characterization of chitosan extracted from Hermetia illucens at different stages of growth.
17. 21^st International Symposium on Bioluminescence and Chemiluminescence & XIX International Symposium on Luminescence Spectrometry. May 31^st-June 3^rd, 2022, Gijon, Spain. Michelini E. – Bioluminescence goes portable: novel biosensors for point-of-need applications.
18. 3^rd International Conference on Analytical and Bioanalytical Methods. October 18-20^th, 2021.Michelini E. – Effect-Based Analysis of Bio-Based Products with Low-Cost Biosensors and Nanotools.
19. 3^rd International Conference on Analytical and Bioanalytical Methods. October18-20^th, 2021. Calabretta M.M. – Paper-Based Biosensors for Global Security Threats.
20. SCI2021 XXVII Congresso Nazionale della Società Chimica Italiana. September 14-23^rd, 2021. Montali L., Lopreside A., Calabretta M.M., Michelini E. – A clover-like paper biosensor for mercury (II) on-site monitoring with a combined bioluminescent-colorimetric detection.
21. Biosensors 2021.July 26-29^th, 2021, Haeundae-gu, South Korea. Montali L., Lopreside A., Wang B., Salinitro M., Tassoni A., Ferri M., Calabretta M.M., Michelini E. – Orthogonal paper biosensor for mercury (II) combining bioluminescence and colorimetric smartphone detection.
22. Innovation in food and wine: a multidisciplinary and synergistic approach to enhance the territory. December 4^th, 2020, Pisa, Italy. Conti B. – PRIMA’s project FEDKITO: Fresh food sustainable packaging in the circular economy.

Webinars:

1. 6^th International Webinar on Chemistry and Pharmaceutical Chemistry, November 24-25^th, 2022. Calabretta M.M. – 3D spherical microtissues: predictive in vitro sensing tools.
2. 180 Years of Excellence in Agricultural Sciences in Pisa: the State of Research Today. October 29^th, 2021, Department of Agriculture, Food and Environment, University of Pisa, Italy. Farina P., Abenaim L., Bedini S., Conti B. – The PRIMA project Fedkito: from insects and plants a green solution to protect fresh fruit and vegetables.
3. 3^rd International Webinar on Chemistry and Pharmaceutical Chemistry. October 29^th, 2021. Calabretta M.M. – Paper-based bio-chemiluminescence biosensors.
4. On-line Symposium on Bioluminescence and Chemiluminescence & XIX International Symposium on Luminescence Spectrometry. June24^th, 2021. Montali L., Lopreside A., Calabretta M.M., Michelini E. – Combined bioluminescent and colorimetric smartphone detection for mercury (II) with a three-leaf paper-based biosensor.
5. Online conference: Pink and green. January 29^th, 2021.

Fairs:

1. EXPO of Dubai. February 17^th – 23^rd, 2022, Dubai, United Arab Emirates. Ranieri A. – FEDKITO project was presented during the Food, Agriculture and Livelihoods Week. https://bit.ly/359ugE0.
2. XIX Festival della Scienza. October 21^st – November 1^st, 2021, Genova, Italy. Ranieri A., Castagna A. – Short video on chitosan extraction and use as edible coating for fresh foods. https://drive.google.com/file/d/1LNWaEy0cewViW31XcTBxhddRI8vaLwA6/view?usp=share_link.

Short videos:

1. FEDKITO short video by Calabretta M.M. and Michelini E. (UNIBO). https://www.youtube.com/watch?v=lKWIz6zEa_4.
2. The chitosan: a precious resource in food preservation by Santin M. (UNIPI). https://drive.google.com/file/d/1xcCVxpIIjQsEBW688kqtGOl14fmj8MNV/view?usp=share_link.

Lectures:

Elisa Michelini (UNIBO) held two lectures tailored for PhD students’ audience:

1. International Biophotonics Artificial Intelligence School in Florence, Italy (September 4-8^th, 2023, http://bpai.ifac.cnr.it).
2. International Biophotonics Artificial Intelligence School in Florence, Italy (September 5-9^th, 2022, http://bpai.ifac.cnr.it).
3. National School of Optical Biosensors and Biophotonic in Ischia, Italy (June 6-10^th, 2022, http://www.na.isasi.cnr.it/scuolabiob4).

Elisa Michelini (UNIBO) held a lecture tailored for graduate students’ audience:

1. International Summer School on Natural Products-2021 (July 16-18^th, 2021, https://www.issnpschool.org).

David Siaussat (SU) held two lectures (October and November 2020) for master students of Ecophysiology and Ecotoxicology, Biodiversity, Ecology and Evolution speaking about the use of essential oils as insecticide or attractant in the Impact of Natural Compounds on pest in M1 level module “ecophysiology” and on M2 level module “Biotic relation in Agronomy”.